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Clicking the pictures or inbound links will redirect you to definitely an internet site hosted by BenchSci that provides third-occasion scientific information. Neither the information nor the BenchSci engineering and processes for choice happen to be evaluated by us; we've been giving them as-is and with no guarantee of any variety, like to be used or application in the Thermo Fisher Scientific goods introduced.Antibodies bound to the blocking peptide not bind to the epitope over the goal protein. By evaluating the staining from your blocked antibody versus the antibody by yourself, one can see which staining is precise.
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This information outlines more possible optimization of mobile and immunoassay parameters to make certain the very best results are...
This manual outlines further more achievable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Clicking the images or one-way links will redirect you to a web site hosted by BenchSci that provides third-party scientific articles. Neither the content nor the BenchSci technology and processes for variety are actually evaluated by us; we're delivering them as-is and with out guarantee of any type, like to be used or application in the Thermo Fisher Scientific solutions introduced.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind precisely on the focus on antibody and block antibody binding. These peptide normally is made up of the epitope acknowledged through the antibody.
Target Information and facts Cyclin E belongs to your extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators website of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a posh with and capabilities like a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S stage boundary and is particularly degraded as cells progress as a result of S period.
Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential role in selling cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been explained. Two extra splice variants have been reported but comprehensive nucleotide sequence information is just not nonetheless accessible.
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